2012

脊椎動物のTRPチャネルによる赤外線感受システム

Gracheva EO, Cordero-Morales JF, Gonzalez-Carcacia JA, Ingolia NT, Manno C, Aranguren CI, Weissman JS, Julius D.(2012) Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats. Nature. 2011 Aug 3;476(7358):88-91.

Gracheva EO, Ingolia NT, Kelly YM, Cordero-Morales JF, Hollopeter G, Chesler AT, Sanchez EE, Perez JC, Weissman JS, Julius D.(2012) Molecular basis of infrared detection by snakes. Nature. 2010 Apr 15;464(7291):1006-11.

ロドプシンの熱活性化メカニズムの理論解析

Gozem S, Schapiro I, Ferre N, Olivucci M.(2012) The molecular mechanism of thermal noise in rod photoreceptors. Science. 2012 Sep 7;337(6099):1225-8.

ショウジョウバエ微絨毛におけるTRP/TRPLチャネルの開閉メカニズム

Roger C. Hardie, Kristian Franze.(2012) Photomechanical Responsese in Drosophila Photoreceptors. Science. 2012 Oct 12;338(6104):260-263.

プロテアーゼ受容体PAR2の呼吸器における生理機能とβアレスチンの関係性

Nichols HL, Saffeddine M, Theriot BS, Hegde A, Polley D, El-Mays T,(2012) b-Arrestin-2 mediates the proinflammatory effects of proteinase-activated receptor-2 in the airway. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16660-5.

セロトニン受容体5-HT2Aと代謝型グルタミン酸受容体mGluR2の多量体化およびその機能に重要なアミノ酸残基の探索

Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cuellar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, Lopez-Gimenez JF, Meana JJ, Benson DL, Gonzalez-Maeso J. (2012) Identification of Three Residues Essential for 5-HT2A-mGlu2 Receptor Heteromerization and its Psychoactive Behavioral Function. J Biol Chem. 2012 Nov 7(Epub).

ナメクジウオの光受容器官に発現する機能分子解析から推定される脊椎動物の眼の進化

Vopalensky P, Pergner J, Liegertova M, Benito-Gutierrez E, Arendt D, Kozmik Z. (2012) Molecular analysis of the amphioxus frontal eye unravels the evolutionary origin of the retina and pigment cells of the vertebrate eye. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15383-8.

クラミドモナスのロドプシンHKR1の光反応の解析

Luck M, Mathes T, Bruun S, Fudim R, Hagedorn R, Nguyen TM, Kateriya S, Kennis JT, Hildebrandt P, Hegemann P. (2012) A photochromic histidine kinase rhodopsin (HKR1) that is bimodally switched by UV and blue light. J Biol Chem. 2012 Oct 1(Epub).

TACEを利用したGPCRのGタンパク質活性化能を測定する新手法

Inoue A, Ishiguro J, Kitamura H, Arima N, Okutani M, Shuto A, Higashiyama S, Ohwada T, Arai H, Makide K, Aoki J. (2012) TGFa shedding assay: an accurate and versatile method for detecting GPCR activation. Nat Methods 2012 Oct;9(10):1021-9.

PYPの光反応による構造変化の解析

Kim TW, Lee JH, Choi J, Kim KH, van Wilderen LJ, Guerin L, Kim Y, Jung YO, Yang C, Kim J, Wulff M, van Thor JJ, Ihee H. (2012) Protein structural dynamics of photoactive yellow protein in solution revealed by pump-probe X-ray solution scattering. J Am Chem Soc. 2012 Feb 15;134(6):3145-53.

Tripathi S, Srajer V, Purwar N, Henning R, Schmidt M. (2012) pH dependence of the photoactive yellow protein photocycle investigated by time-resolved crystallography. Biophys J. 2012 Jan 18;102(2):325-32.

錐体視細胞に存在するRPE65の機能推定

Tang PH, Buhusi MC, Ma JX, Crouch RK. (2011) RPE65 is present in human green/red cones and promotes photopigment regeneration in an in vitro cone cell model. J Neurosci. 2011 Dec 14;31(50):18618-26.

Tang PH, Wheless L, Crouch RK. (2011) Regeneration of photopigment is enhanced in mouse cone photoreceptors expressing RPE65 protein. J Neurosci. 2011 Jul 13;31(28):10403-11.

海馬にチャネルロドプシンChR2を発現させることによる記憶の光誘起

Liu X, Ramirez S, Pang PT, Puryear CB, Govindarajan A, Deisseroth K, Tonegawa S. (2012) Optogenetic stimulation of a hippocampal engram activates fear memory recall. Nature 2012 Mar 22;484(7394):381-5.

CNGチャネルの欠損による小胞体ストレスが錐体視細胞の変性を引き起こす

Thapa A, Morris L, Xu J, Ma H, Michalakis S, Biel M, Ding XQ. (2012) Endoplasmic Reticulum Stress-associated Cone Photoreceptor Degeneration in Cyclic Nucleotide-gated Channel Deficiency. J Biol Chem. 2012 May 25;287(22):18018-29.

視細胞の移植による視覚機能の回復

Pearson RA, Barber AC, Rizzi M, Hippert C, Xue T, West EL, Duran Y, Smith AJ, Chuang JZ, Azam SA, Luhmann UF, Benucci A, Sung CH, Bainbridge JW, Carandini M, Yau KW, Sowden JC, Ali RR. (2012) Restoration of vision after transplantation of photoreceptors. Nature 2012 May 3;485(7396):99-103.

リガンドやシグナルタンパク質の結合によるGPCRの構造アンサンブルの変化の観察

Mary S, Damian M, Louet M, Floquet N, Fehrentz JA, Marie J, Martinez J, Baneres JL. (2012) Ligands and signaling proteins govern the conformational landscape explored by a G protein-coupled receptor. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8304-9.

心臓におけるプロスタグランジンE受容体によるGs系シグナル伝達機構の阻害メカニズム

Liu S, Li Y, Kim S, Fu Q, Parikh D, Sridhar B, Shi Q, Zhang X, Guan Y, Chen X, Xiang YK. (2012) Phosphodiesterases coordinate cAMP propagation induced by two stimulatory G protein-coupled receptors in hearts. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6578-83.

シグナル伝達系駆動偏向性リガンドのGPCR活性化における分子メカニズム

Rahmeh R, Damian M, Cottet M, Orcel H, Mendre C, Durroux T, Sharma KS, Durand G, Pucci B, Trinquet E, Zwier JM, Deupi X, Bron P, Baneres JL, Mouillac B, Granier S. (2012) Structural insights into biased G protein-coupled receptor signaling revealed by fluorescence spectroscopy. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6733-8.

肝臓がん細胞におけるOPN3の発現およびアポトーシスへの影響

Jiao J, Hong S, Zhang J, Ma L, Sun Y, Zhang D, Shen B, Zhu C. (2012) Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating the apoptotic pathway. Cancer Lett. 2012 Jul 1;320(1):96-103.

beta2アドレナリン受容体およびGsの構造変化・揺らぎの観測

Chung KY, Rasmussen SG, Liu T, Li S, DeVree BT, Chae PS, Calinski D, Kobilka BK, Woods VL Jr, Sunahara RK. (2011) Conformational changes in the G protein Gs induced by the beta2 adrenergic receptor. Nature. 2011 Sep 28;477(7366):611-5.

Bockenhauer S, Furstenberg A, Yao XJ, Kobilka BK, Moerner WE. (2011) Conformational dynamics of single G protein-coupled receptors in solution. J Phys Chem B. 2011 Nov 17;115(45):13328-38.

チャネルロドプシンChR2を用いた大脳皮質の視覚野のL6層のはたらきの解析

Olsen SR, Bortone DS, Adesnik H, Scanziani M. (2012) Gain control by layer six in cortical circuits of vision. Nature. 2012 Feb 22;483(7387):47-52.

線虫を用いたGPCR発現の実験系の開発

Salom D, Cao P, Sun W, Kramp K, Jastrzebska B, Jin H, Feng Z, Palczewski K. (2012) Heterologous expression of functional G-protein-coupled receptors in Caenorhabditis elegans. FASEB J. Epub 2011 Nov 16.

ショウジョウバエの概日リズム調節にRh5とRh6が関わる

Szular J, Sehadova H, Gentile C, Szabo G, Chou WH, Britt SG, Stanewsky R. (2012) Rhodopsin 5- and Rhodopsin 6-Mediated Clock Synchronization in Drosophila melanogaster is Independent of Retinal Phospholipase C-β Signaling. J Biol Rhythms. Epub 2012 Feb 27.

ショウジョウバエの偏光視に関する実験

Wernet MF, Velez MM, Clark DA, Baumann-Klausener F, Brown JR, Klovstad M, Labhart T, Clandinin TR. (2012) Genetic dissection reveals two separate retinal substrates for polarization vision in Drosophila. Curr Biol. Epub 2011 Dec 15.

Weir PT, Dickinson MH. (2012) Flying Drosophila orient to sky polarization. Curr Biol. Epub 2011 Dec 15.

ショウジョウバエの視細胞におけるRh1とTRPの品質管理機構

Rosenbaum EE, Brehm KS, Vasiljevic E, Liu CH, Hardie RC, Colley NJ. (2011) XPORT-Dependent Transport of TRP and Rhodopsin Neuron. Epub 2011 Nov 17.

虹彩に発現しているメラノプシンによる瞳孔反射の制御

Xue T, Do MT, Riccio A, Jiang Z, Hsieh J, Wang HC, Merbs SL, Welsbie DS, Yoshioka T, Weissgerber P, Stolz S, Flockerzi V, Freichel M, Simon MI, Clapham DE, Yau KW. (2011) Melanopsin signalling in mammalian iris and retina. Nature. Epub 2011 Nov 2.

視交叉上核内の概日リズムの決定と光情報変換に関わるNav1.1チャネルの役割

Han S, Yu FH, Schwartz MD, Linton JD, Bosma MM, Hurley JB, Catterall WA, de la Iglesia HO.　(2012) NaV1.1 channels are critical for intercellular communication in the suprachiasmatic nucleus and for normal circadian rhythms. Proc Natl Acad Sci U S A. Epub 2012 Jan 5.

セロトニン受容体・代謝型グルタミン酸受容体のヘテロ二量体化によるGタンパクの活性制御と精神病との関連

Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, MacKerell AD Jr, Brezina V, Sealfon SC, Filizola M, Gonzalez-Maeso J, Logothetis DE. (2011) Decoding the signaling of a GPCR heteromeric complex reveals a unifying mechanism of action of antipsychotic drugs. Cell. 2011 Nov 23;147(5):1011-23.

Gonzalez-Maeso J, Ang RL, Yuen T, Chan P, Weisstaub NV, Lopez-Gimenez JF, Zhou M, Okawa Y, Callado LF, Milligan G, Gingrich JA, Filizola M, Meana JJ, Sealfon SC. (2011) Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature. Epub 2008 Feb 24.

網膜色素変性症または先天停止性夜盲に関わるロドプシンG90変異体の分子メカニズム

Toledo D, Ramon E, Aguila M, Cordomi A, Perez JJ, Mendes HF, Cheetham ME, Garriga P. (2011) Molecular mechanisms of desease for mutations at Gly-90 in rhodopsin. J Biol Chem. Epub 2011 Sep 22.