2013

メラトニン受容体のヘテロ二量体の機能と視細胞応答への影響

Heteromeric MT1/MT2 melatonin receptors modulate photoreceptor function
Baba K, Benleulmi-Chaachoua A, Journé AS, Kamal M, Guillaume JL, Dussaud S, Gbahou F, Yettou K, Liu C, Contreras-Alcantara S, Jockers R, Tosini G.
Sci Signal 2013 Oct 8;6(296)

桿体の単一光子応答とDark noiseに関する電気生理・シミュレーション実験

Detection of single photons by toad and mouse rods.
Reingruber J, Pahlberg J, Woodruff ML, Sampath AP, Fain GL, Holcman D.
PNAS 2013 Nov 26;110(48):19378-83

ipRGCの明順応

Adaptation to steady light by intrinsically photosensitive retinal ganglion cells.
Do MT, Yau KW.
PNAS 2013 Apr 30;110(18):7470-5.

温度依存的に構造変化するタンパク質をGFPに融合し細胞内温度変化を観測する新たな手法について

Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells
Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu-Kanbe M, Matsuura H, Imamura H, Yoshizaki T, Hamachi I, Morii T, Mori Y.
Nat Methods 2013 Oct 13

自由運動するマウスの筋肉を光遺伝学的に制御

Optogenetic control of targeted peripheral axons in freely moving animals
Towne C, Montgomery KL, Iyer SM, Deisseroth K, Delp SL.
PLoS One 2013 Aug 21;8(8)

メラノプシンの活性を特異的に抑制する小分子

Small-molecule antagonists of melanopsin-mediated phototransduction
Jones KA, Hatori M, Mure LS, Bramley JR, Artymyshyn R, Hong SP, Marzabadi M, Zhong H, Sprouse J, Zhu Q, Hartwick AT, Sollars PJ, Pickard GE, Panda S.
Nat Chem Biol 2013 Oct;9(10):630-5

アレスチンの活性構造について

Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide
Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ
Nature 2013 May 2;497(7447):137-41

Crystal structure of pre-activated arrestin p44
Kim YJ, Hofmann KP, Ernst OP, Scheerer P, Choe HW, Sommer ME
Nature2013 May 2;497(7447):142-6

ショウジョウバエのクリプトクロムと視覚系について

Fly cryptochrome and the visual system
Gabriella Mazzotta, Alessandro Rossi, Emanuela Leonardi, Moyra Mason, Cristiano Bertolucci, Laura Caccin, Barbara Spolaore, Alberto J. M. Martin, Matthias Schlichting, Rudi Grebler, Charlotte Helfrich-Förster, Stefano Mammi, Rodolfo Costa, Silvio C. E. Tosatto
Proc Natl Acad Sci U S A 2013 April 9; 110(15): 6163–6168.

脊椎動物脳内で介在ニューロン・運動ニューロンにVAL・TMTオプシンが共発現している

Co-Expression of VAL- and TMT-Opsins Uncovers Ancient Photosensory Interneurons and Motorneurons in the Vertebrate Brain.
Fischer RM, Fontinha BM, Kirchmaier S, Steger J, Bloch S, Inoue D, Panda S, Rumpel S, Tessmar-Raible K.
PLoS Biol 　2013 Jun;11(6).

ロドプシンダイマーの非対称性：Gtとの複合体＆Arrestinとの複合体

The rhodopsin-transducin complex houses two distinct rhodopsin molecules.
Jastrzebska B, Ringler P, Palczewski K, Engel A.
J Struct Biol 　2013 May;182(2):164-72.

Distinct loops in arrestin differentially regulate ligand binding within the GPCR opsin.
Sommer ME, Hofmann KP, Heck M.
Nat Commun 2012;3:995

「塩味の感じ方」について

High salt recruits aversive taste pathways.
Oka Y, Butnaru M, von Buchholtz L, Ryba NJ, Zuker CS.
Nature 2013 Feb 28;494(7438):472-5.

tmc-1 encodes a sodium-sensitive channel required for salt chemosensation in C. elegans.
Chatzigeorgiou M, Bang S, Hwang SW, Schafer WR.
Nature 2013 Feb 7;494(7435):95-9

ER/Kリンカーで結合した蛍光タンパク質を用いたセンサーおよびGPCRへの応用

Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, Sivaramakrishnan S.(2013) Detection of G-protein selective G-protein Coupled Receptor (GPCR) conformations in live cells. J Biol Chem. 2013 Apr 29. (Epub)

Sivaramakrishnan S, Spudich JA.(2011) Systematic control of protein interaction using a modular ER/K α-helix linker. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20467-72.

ゼブラフィッシュ胚における光依存的非視覚行動の分子・細胞的メカニズムの解析

Kokel D, Dunn TW, Ahrens MB, Alshut R, Cheung CY, Saint-Amant L, Bruni G, Mateus R, van Ham TJ, Shiraki T, Fukada Y, Kojima D, Yeh JR, Mikut R, von Lintig J, Engert F, Peterson RT.(2013) Identification of nonvisual photomotor response cells in the vertebrate hindbrain. J Neurosci. 2013 Feb 27;33(9):3834-43.

βアレスチンを介したGPCRのシグナル伝達メカニズムの解明

Wehbi VL, Stevenson HP, Feinstein TN, Calero G, Romero G, Vilardaga JP.(2013) Noncanonical GPCR signaling arising from a PTH receptor-arrestin-Gβγ complex. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1530-5.

Li B, Wang C, Zhou Z, Zhao J, Pei G.(2013) β-Arrestin-1 directly interacts with Gαs and regulates its function. FEBS Lett. 2013 Mar 1;587(5):410-6.

ロドプシンを応用した実験技術

Barish PA, Xu Y, Li J, Sun J, Jarajapu YP, Ogle WO.(2013) Design and functional evaluation of an optically active μ-opioid receptor. Eur J Pharmacol. 2013 Feb 27;705(1-3):42-48.

Maclaurin D, Venkatachalam V, Lee H, Cohen AE.(2013) Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44.

微生物の免疫システムであるCRISPR/Casシステムを用いた遺伝子操作

Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F.(2013) Multiplex genome engineering using CRISPR/Cas systems. Science. 2013 Feb 15;339(6121):819-23.

Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM.(2013) RNA-guided human genome engineering via Cas9. Science. 2013 Feb 15;339(6121):823-6.

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E.(2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012 Aug 17;337(6096):816-21.

クシクラゲのカルシウム受容発光タンパク質Photoproteinおよびオプシンの系統解析

Schnitzler CE, Pang K, Powers ML, Reitzel AM, Ryan JF, Simmons D, Tada T, Park M, Gupta J, Brooks SY, Blakesley RW, Yokoyama S, Haddock SH, Martindale MQ, Baxevanis AD.(2012) Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: a new view of ctenophore photocytes. BMC Biol. 2012 Dec 21;10:107.

熱帯縞蚊における光依存的なAaop1の局在変化

Hu X, Leming MT, Metoxen AJ, Whaley MA, O'Tousa JE.(2012) Light-mediated control of rhodopsin movement in mosquito photoreceptors. J Neurosci. 2012 Oct 3;32(40):13661-7

内因性光感受性網膜神経節細胞(ipRGC)が光依存的に気分や学習に影響を与えている

LeGates TA, Altimus CM, Wang H, Lee HK, Yang S, Zhao H, Kirkwood A, Weber ET, Hattar S.(2012) Aberrant light directly impairs mood and learning through melanopsin-expressing neurons. Nature. 2012 Nov 22;491(7425):594-8.

LOVドメインおよびBLUFドメインの光による構造変化およびオプトジェネティクスへの応用

Christie JM, Gawthorne J, Young G, Fraser NJ, Roe AJ.(2012) LOV to BLUF: flavoprotein contributions to the optogenetic toolkit. Mol Plant. 2012 May;5(3):533-44.

Mitra D, Yang X, Moffat K.(2012) Crystal structures of Aureochrome1 LOV suggest new design strategies for optogenetics. Structure. 2012 Apr 4;20(4):698-706.

代謝型グルタミン酸受容体mGluRの活性化メカニズムの解析

El Moustaine D, Granier S, Doumazane E, Scholler P, Rahmeh R, Bron P, Mouillac B, Baneres JL, Rondard P, Pin JP.(2012) Distinct roles of metabotropic glutamate receptor dimerization in agonist activation and G-protein coupling. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16342-7.

Hlavackova V, Zabel U, Frankova D, Batz J, Hoffmann C, Prezeau L, Pin JP, Blahos J, Lohse MJ.(2012) Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1. Sci Signal. 2012 Aug 14;5(237):ra59.